lnd - ba 1k addressable biosensor arrays data sheet d general description ? microarray of electrodes i2c or spi addressable ? electrical control for each array element and segment ? mux up to four high impedance input lines i nto array or; ? read array ? 2240 array sites features the custom oligonucleotide microarray is synthesized using a semiconductor - based electrochemical - synthesis process. each oligonucleotide probe is synthesized via a platinum electrode that is independently controlled by the synthesize rs computer. synthesis is based on established phosphoramidite chemistry and occurs at thousands of sites simultaneously according to a computer algorithm that activates only specified electrodes. since physical photolithographic masks or pre - built collec tions of oligos are not involved in the process, all probes can be easily changed without extra time or cost. applications ? mr na ? rna ? snp genotyping ? re - sequencing custom array concept ? linear dimensions semiconduct or inc. ? 22620 ricardo rd, cupertino ca 95014 usa ? tel 312.321.1810 ? fax 312.321.1830 ? www.lineardimensions.com ?
chemistry of in - situ oligonucleotide synthesis on microelectrodes. the linear dimensions chips are produced in a form that the protected thymines are attached on the surface of each electrode. by computer - controlled electronics, selected electrodes are turned onto form effective a cidic local environment around the corresponding electrodes. the protection groups on thymines are cleaved off in the acidic environment. microfluidic device supplies amidite solutions which couples to the deprotected nucleotide to form phosphodiester bon d. the chip surface is washed to remove any free amidite on the solution. the repetition of selected deprotection - couple - wash step results in the synthesis of oligonucleotides with custom - designed sequence. lnd - ba1k ? linear dimensions semiconductor inc. ? 22620 ricardo rd, cupertino ca 95014 us a ? tel 312.321.1810 ? fax 312.321.1830 ? www.lineardimensions.com ?
two platform shares the in - situ nature of the oligonucleotide synthesis, but they differ in the principle of deprotecting the protecting group of amidites. in contrast linear dimensions where the electric signal control s the deprotection chemistry. affymetrix chip uses photolithographic principle where the photomasks are used to selectively illuminate the position where the next coupling should be performed. the synthesis of affymetrix chip needs set of photomasks designed and synthesized before the actual synthesis of the chip. thus, the change of custom design is more time - and resource - consuming compared to linear dimensions chip where the only thing you need to do is to change the sequence file for the synthesi s software. moreover, the state - of - the - art microfulics design and electronic controls enables the exact synthesis chemistry that linear dimensions offers precise synthesis of oligonucleotide up to 50 - mer, compared to 25 - mer of affymetrix. affymetrix lin ear dimensions photolithography controlled synthesis electronics controlled synthesis needs to produce masks for each base sequence software controlled sequence up to 25 - mer up to 50 - mer lnd - ba1k c omparison between linear dimensions and affymetrix chips ? linear dimensions semiconductor inc. ? 22620 ricardo rd, cupertino ca 95014 usa ? tel 312.321.1810 ? fax 312.321.1830 ? www.lineardimensions.com ?
the stripping of linear dimensions chip is bas ed on chemical denaturation of dna:dna and dna:rna hybrids. the stripping enables for users to perform hybridization up to 3 times. probes that are longer than 40 - mer may not be stripped completely. optimized for dna and rna targets labeled with biotin, a nd with cy5. microarrays must be kept wet for re - usage. the figures 1 - 3 illustrate the efficiency of stripping. lnd - ba1k c ustomarray tm chips are re - usable ? linear dimensions semiconductor inc. ? 22620 ricardo rd, cupertino ca 95014 usa ? tel 312.321.1810 ? fax 312.321.1830 ? www.lineardimensions.com ? re - usable custom array
lnd - ba1k stripping kit die image ? linear dimensions se miconductor inc. ? 22620 ricardo rd, cupertino ca 95014 usa ? tel 312.321.1810 ? fax 312.321.1830 ? www.lineardimensions.com ?
pin number description 1 feedback 2 v0 3 v1 4 v2 5 v3 6 iref 7 gnd 8 clock 9 ss 10 mosi 11 miso 12 vdd ? linear dimensions semiconductor inc. ? 22620 ricardo rd, cupertino ca 95014 usa ? tel 312.321.1810 ? fax 312.321.1830 ? www.lineardimensions.com ? lnd - ba1k pin description layout
lnd - ba1k orientation options ? linear dimensions semiconductor inc. ? 22620 ricardo rd, cupertino ca 95014 usa ? tel 312.321.1810 ? fax 312.321.1830 ? www.lineardimensions.com ?
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