assay characteristics hnpcc mastr plus the hnpcc mastr plus is a molecular research assay for the identification of variants in 4 genes ( mlh1, msh2, msh6 , and? pms2 ) and?3 utr ? of? epcam , which are associated with hereditary non-polyposis colorectal cancer (hnpcc). this assay is ready to use and offers robust performance with minimum hands-on time. it consists of all the reagents necessary to enable multiplex amplification of 84 amplicons for complete exon coverage of the? mlh1, msh2, msh6, pms2 and 3 utr ? of? epcam . research application ? for detection of germline variants, snvs and cnvs, in mlh1, msh2, msh6 , and? pms2 and?3 utr?of? epcam in blood-derived dna. genes full coding region of mlh1, msh2, msh6, and?pms2 and?3 utr ?of?epcam genomic region analyzed 20 kb number of amplicons 84 amplicon length 277-443 bp number of plexes 5 designed to be compatible with miseq performance characteristics * only 192 mid combinations available. uniformity of amplifcation (0.2x mean coverage) 100 % on target read count > 95 % dna input as low as 20 ng per plex reaction number of samples/run (20 reads/allele): illumina miseq v2: 670* illumina miseq v3: 1,229* graph presenting the read pair counts for all hnpcc mastr plus amplicons, showing their outstanding uniform representation. to allow comparison between samples, the read pair counts were normalized to coverage of 100. the data are based on a 10 samples study, using the illumina miseq? system. 10 30 50 70 90 110 130 150 170 190 210 230 250 270 290 310 330 350 370 390 410 430 450 470 490 0% 2% 4% 6% 8% 10% 12% 14% 16% 20% 18% percentage of amplicons read pair counts
order information cat. no. product name reactions mr-0031.024 hnpcc mastr plus 25 mid (molecular identifiers) kits are necessary to complete the workflow. for research use only. not for use in diagnostic procedures. multiplicom n.v. [ a ] galile?laan 18, b-2845 niel, belgium [ w ] www.agilent.com [ e ] mtp.customerservice@agilent.com [ t ] +32 3 289 14 00 [ f ] +32 3 400 23 16 workflow plan run multiplex pcr quality control universal pcr quality control equimolar pooling mixing plexes of single sample puri? cation puri? cation multiplex pcr dilution universal pcr clean up mixing clean up pooling sequencing data analysis mastr plus work ow ngs ? agilent technologies, inc. 2017 printed in belgium, december 31, 2017 5991-8388enn pr7000-1413
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